In vitro biological evaluations

Principal investigators: Silvia Panseri, Monica Montesi

Personnel involved: Giada Bassi, Aurora Grimaudo

The activity of the Cell-Material BioLAB is focused on the study of cell-material interaction by means of cellular and molecular biology techniques. Accuracy is the main pillar of the Lab, that is extremely important to reach the goal of the translational research to the preclinical study and to the medical use.

The laboratory of Cell-Material BioLAB aims to evaluate the biocompatibility and biofunctionality of novel biomaterials (3D scaffolds, micro- and nanoparticles, films, hydrogels, etc.) for regenerative medicine and nanomedicine.

Mammalian cell lines and primary cells (differentiated or stem cells), isolated from different tissues, are used to evaluate proliferation, adhesion, vitality, apoptosis, extracellular matrix protein synthesis activity, growth factors, inflammatory cytokines and enzymes.

Two main macro-areas of interest are: Tissue engineering and nanomedicine.

Tissue engineering
In tissue engineering studies, 3D biomaterials are analyzed in vitro looking at the cell colonization and cell differentiation together with the neo-formed tissue according to chemical and structural characteristics of scaffolds. Biomaterials are also studied also in association to biological stimulators (differentiated and dedifferentiated cells, growth factors and other signal molecules, biophysical stimulations). A perfusion bioreactor gives us the possibility to create in vitro a 3D engineered construct mimicking physiological conditions.

Nanomedicine
Nanostructured materials (e.g. novel nanoparticles) are study as innovative biomolecules delivery system (e.g. drugs, growth factors, miRNA). Real Time analysis gives us the possibility to understand how they interact with the cell membrane and follow the system inside the cells. Biophysical stimulations, like electromagnetic fields, are used for remote delivery.

Further aims of the lab are centered on excellence:

  • Innovation, develop ad hoc systems/protocols for the biological study of biomaterials.
  • Increase of the know-how of the cell-material interaction
  • Training of young researchers in nanomedicine
  • Internationality, attract foreign researchers and/or establish international collaborations
  • Offer activities to the biomedical enterprises (SMEs)

Equipment and processes

  • In vitro evaluation of cytotoxicity and bioactivity of new biomaterials
  • 3D cell cultures with innovative biomaterials in static and dynamic conditions
  • Analysis of cell viability, morphology, proliferation, gene and protein expression (Advanced fluorescence inverted microscope with TimeLapse, Real Time PCR, Western Blot)

 

Cell/biomaterial interaction

Main collaborations

  • Università di Messina (Dr.ssa A. Piperno; Dr.ssa A. Scala)
  • Univ. Polytechnique Hauts-de-France, Laboratoire des Matériaux Céramiques et Procédés Associés, Valenciennes, France (Dr. A. Leriche)
  • University of Chemical Technology and Metallurgy, Department of Organic Chemistry (Dr. Ivan Ivanov)
  • Department of Chemistry, Maynooth University,Dublin, Ireland (Dr. D. Montagner).

Projects

Publications & Patents

  • Fernandes Patrício TM, Panseri S, Montesi M, Iafisco M, Sandri M, Tampieri A, Sprio S. Superparamagnetic hybrid microspheres affecting osteoblasts behaviour. Mater Sci Eng C Mater Biol Appl. 2019 Mar;96:234-247. doi: 10.1016/j.msec.2018.11.014
  • Tampieri A, Ruffini A , Ballardini A , Montesi M , Panseri S , Salamanna F , Fini M , Sprio S . Heterogeneous chemistry in the 3-D state: An original approach to generate bioactive, mechanically-competent bone scaffolds. Biomater Sci. 2018 Dec 18;7(1):307-321. doi: 10.1039/c8bm01145a.
  • Montesi M, Panseri S, Dapporto M, Tampieri A, Sprio S. Sr-substituted bone cements direct mesenchymal stem cells, osteoblasts and osteoclasts fate. PLoS One. 2017 Feb 14;12(2):e0172100. doi: 10.1371/journal.pone.0172100
  • Neri G, Micale N, Scala A, Fazio E, Mazzaglia A, Mineo P.G, Montesi M, Panseri S, Tampieri A, Grassi G, Piperno A. Silibinin-conjugated graphene nanoplatform: Synthesis, characterization and biological evaluation. FlatChem January 2017, 34–41.
  • Campodoni E, Adamiano A, Dozio SM, Panseri S, Montesi M, Sprio S, Tampieri A, Sandri M. Development of Innovative Hybrid and Intrinsically Magnetic Nanobeads as Drug Delivery. Nanomedicine (Lond.). August 2016. 11(16): 2119-2130. doi:10.2217/nnm-2016-0101
  • Panseri S, Montesi M, Dozio SM, Savini E, Tampieri A and Sandri M. Biomimetic scaffold with Aligned Microporosity Designed for Dentin Regeneration. 2016 Front. Bioeng. Biotechnol. 4:48. doi: 10.3389/fbioe.2016.00048
  • Panseri S, Montesi M., Sandri M, Iafisco M, Adamiano A, Ghetti M, Cenacchi C and Tampieri A. Magnetic labelling of mesenchymal stem cells with iron-doped hydroxyapatite nanoparticles as tool for cell therapy. J Biomed Nanotechnol. 2016. 12, 909-921.doi:10.1166/jbn.2016.2248.
  • Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A. Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis. PLoS One. 2015 Jul 6. doi: 10.1371/journal.pone.0132633.